Fig. 2

Plasmid combinations used in this study for whole-cell biocatalyst design. The P450 gene cyp105D is always encoded on the pET22b-vector. Redox partner genes (pdx/pdr) are integrated in MCSI of pCOLADuet. The re-adh gene (b) is integrated in the MCSII of the pCOLADuet-vector. Gray squares represent the T7-promoter; gray circles indicate the ribosome binding site