Fig. 1From: Evaluation of P450 monooxygenase activity in lyophilized recombinant E. coli cells compared to resting cellsSchematic overview of the whole-cell biocatalyst expressing CYP105D from S. platensis for the oxidation of testosterone 1. Putidaredoxin reductase (Pdr) and putidaredoxin (Pdx) from P. putida are used as redox partners for CYP105D. Alcohol dehydrogenase (ADH) from R. erythropolis was implemented for cofactor regeneration using propan-2-ol as sacrificial substrate and solvent for testosterone solubilizationBack to article page