Fig. 5
The amount of alkaline phosphatase measured in the supernatants from P. aeruginosa PAO1 at biofilm and planktonic states, respectively. The amount of alkaline phosphatase was defined as the µmoles of p-nitrophenol liberated from p-nitrophenyl phosphate at the specific time and the data was normalized to log10CFU. Data were collected from three independent experiments with at least three replicates each. Error bars represent the standard deviation from three independent experiments. P values that are significantly different by T.test are indicated by asterisks as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001