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Table 5 The characteristics of fungal cellobiohydrolases

From: Recombinant cellobiohydrolase of Myceliophthora thermophila: characterization and applicability in cellulose saccharification

Protein

GH family

Source organism

Molecular mass (kDa)

Enzyme activity

Optimum pH and Temp. (°C)

Enzyme kinetics

Reference

MtCel6A

6

Myceliophthora thermophila

 ~ 45

172 U mg−1

10 and 60

Km = 3.2 mg mL −1,

Vmax = 222.2 μM mg −1 min −1

This investigation

TlCel6A

6

Talaromyces leycettanus

65

92.9 U mg−1

5 and 80

NR

Gu et al. 2019

Ctcel6

6

Chaetomium thermophilum

42

1.27 U mg−1

5 and 70

Km = 0.30 mM

Zhou et al. 2017

CelA

6

Neocallimastix patriciarum J11

55

200 U mg−1

6 and 50

NR

Wang et al. 2014

CBH1 and CBH2

NR

Schizophyllum commune KMJ820

50 and 150

19.57and 18.12

U mg−1

5 and 50

Km = 2.0 mM and 1.4 mM

Vmax = 51.4 and 20.8 U mg−1

Kondaveeti et al. 2020

CtCel7

7

Chaetomium thermophilum

73

1.94 U mg−1

4 and 60

Km = 4 mg mL−1,

Vmax = 18.45 ug min−1 mL−1

kcat/Km = 391 mL s−1 mg−1

Han et al. 2020

CBHI

7

Penicillium digitatum

74

30.9 U mg−1

5.2 and 60

Km = 11.2 mg mL−1,

Vmax = 0.13 μmol min −1,

kcat/Km = 4.7 (mg mL−1)−1 s−1

Dos Santos et al. 2020

Cbh1

7

Trichoderma virens UKM1

54

4.195 U mg−1

4 and 60

Km = 1.88 mM

Vmax = 41.6 µmole min−1 mg−1

kcat/Km = 5.68 × 10−4 mM−1 s−1

Wahab et al. 2019

Te-Cel7A

7

Trichoderma reesei

66

0.93 U L−1

5 and 65

NR

Sun et al. 2018

MtCel7A

7

Myceliophthora thermophila

66

6.8 U mg−1

4–5 and 70

Km = 0.57 mM,

kcat/Km = 6.7 M−1 s−1

Kadowaki et al. 2018

CBH7B

7

Thielavia terrestris

51.8

63 U mg−1

5 and 55

Km = 0.28 mM

Vmax = 402 nmol min−1 mg−1

kcat = 0.35 s−1

Woon et al. 2016

  1. NR Not reported