Table 3 Effect of various additives on rMtCel6A activity
Additives | Residual rMtCel6A activity (%) | Additives | Residual rMtCel6A activity (%) |
|---|---|---|---|
Control | 100 ± 1.09 | Control | 100 ± 1.09 |
Metal ions | 5 mM | Surfactants | 0.5% |
Na+ Ca2+ Co2+ K+ Mg2+ Mn2+ Fe2+ Cu2+ Zn2+ NH+ | 90 ± 1.20 88 ± 0.90 113 ± 1.24 94 ± 1.31 97 ± 0.90 114 ± 1.40 84 ± 1.52 110 ± 0.79 81 ± 0.99 89 ± 2.01 | SDS Tween-80 Triton X-100 CTAB PEG | 86 ± 2.09 67 ± 1.97 91 ± 1.76 78 ± 1.39 80 ± 1.80 |
Organic solvents | 10% | Modulators | 5 mM |
Acetone Butanol Chloroform Ethanol Ethyl acetate Hexane Isoamyl-alcohol Iso-propanol Liquor ammonia Methanol | 137 ± 1.67 100 ± 1.73 94 ± 1.70 103 ± 0.96 ND 118 ± 1.29 133 ± 1.87 98 ± 1.57 120 ± 1.26 100 ± 1.05 | IA DEPC DTT EDAC EDTA NBS PMSF WRK | 50 ± 1.80 70 ± 1.69 60 ± 0.97 40 ± 1.54 62 ± 1.04 45 ± 1.45 75 ± 1.28 25 ± 2.01 |