Fig. 4From: Recombinant cellobiohydrolase of Myceliophthora thermophila: characterization and applicability in cellulose saccharificationa Purification and zymogram of rMtCel6A. M: protein marker, L1: purified rMtCel6A, L3: zymogram showing fluorescence [Protein ladder (PageRuler Plus prestained) was procured from Thermo Scientific] b Qualitative cellobiohydrolase assay of various fractions of rMtCel6A using 4-MUC as substrate, where well 1: negative control (pH 10 buffer only), well 2: supernatant fraction obtained after lysis of rMtCel6A; well 3: rMtCel6A pure IBs (unfolded); well 4: solubilized and refolded IBs of rMtCel6ABack to article page