Fig. 3
From: Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4
Identification of antigenic epitope in Hexon of FAdV4 recognized by mAb 3G8. SDS-PAGE (a) and WB (b) analysis of the expression of truncated Hexon proteins. A series of hexon fragments were cloned into pET-30a (+) and expressed in E. coli. Antigenic epitope of mAb 3G8 was located between hexon657 and hexon540. 1, empty vector;2, hexon879; 3, hexon798;4, hexon750; 5, hexon657; 6, hexon540; 7, hexon375; lane 8, hexon459; lane 9, hexon405; 10, hexon312. SDS-PAGE (c) and WB (d) analysis of the expression of truncated Hexon proteins. A series of hexon fragments were cloned into pET-30a (+) and expressed in E. coli. An epitope of “AYGAYVK” located between hexon636 and hexon657 was characterized as the antigenic epitope recognized by mAb 3G8. 1, hexon540; 2, hexon570; 3, hexon582; 4, hexon600; 5, hexon615; 6, hexon636; 7, hexon657; 8, hexon879; 9, hexon601-879.