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Fig. 1 | AMB Express

Fig. 1

From: Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4

Fig. 1

Characterization of the generated mAb 3G8. a The N-terminus of Hexon peptide (1-293aa) of FAdV-4 was expressed and analyzed by SDS-PAGE. 1, empty vector; 2, non-induced BL21 (DE3) transformed with pET30a (+)-hexon879; 3, purified Hexon protein; 4, induced BL21 (DE3) transformed with pET30a (+)-hexon879. b The mAb 3G8 reacted with the Hexon protein expressed in cell by IFA. pcDNA3-hexon879 plasmid was transfected to LMH cells. At 70 h post-transfection, the cells were fixed and incubated with mAb 3G8 for 30 min. Following three washes with PBS, the cells was incubated with FITC-conjugated anti-mouse antibody and examined under an Axiophot Photo microscope

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