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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Histamine binding activity of surface-modified yeast by histamine binding protein (HBP)

Fig. 2

Western blot was performed to confirm expression of the recombinant protein. Cells were sonicated and separated into soluble and insoluble proteins. Since HBP was expressed on the cell surface, it is an insoluble protein, and the protein size of recombinant protein is 29.4 kDa. As a control, GWL-0 cells were used expressing a yeast surface protein (Cwp2p) without the HBP gene. As shown in the Fig. 4, a band of 29.4 kDa is seen in the insoluble protein of the GWL-1 strain

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