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Table 1 Summary of macroscopic data for the strains Re2133/pCB1 (with or without kanamycin; with pulse or continuous fructose feeding) and pCB3

From: Investigation of the robustness of Cupriavidus necator engineered strains during fed-batch cultures

Strains \(\mu\) RS/X RNH3/X RS/CO2 By-products RQ qCO2 qO2
(h−1) (gX·gS−1) (gX·gNH3−1) (gCO2·gS−1)    (mmol·gX−1·h−1) (mmol·gX−1·h−1)
Re2133/pCB1 + Kan
Pulse feeding
0.25 ± 0.01 0.28 ± 0.01 6.6 ± 0.2 0.74 ± 0.01 acetate, succinate, aceto-acetate, citrate 1.12 ± 0.08 2.41 ± 0.30 2.14 ± 0.22
Re2133/pCB1 + Kan
Continuous feeding
0.22 ± 0.01 0.32 ± 0.01 5.4 ± 0.2 0.85 ± 0.02 none 1.13 ± 0.03 2.81 ± 0.57 2.48 ± 0.49
Re2133/pCB1
Continuous feeding
0.24 ± 0.01 0.32 ± 0.01 5.60 ± 0.08 0.89 ± 0.02 none 1.05 ± 0.06 2.14 ± 0.23 2.05 ± 0.24
Re2133/pCB3
Continuous feeding
0.20 ± 0.01 0.32 ± 0.01 5.21 ± 0.41 0.87 ± 0.02 none 1.14 ± 0.02 2.10 ± 0.40 1.84 ± 0.30
Reference (Aragao et al. 1996)   0.53 5.96 0.51