Fig. 3
From: Investigation of the robustness of Cupriavidus necator engineered strains during fed-batch cultures
Relative extracellular fluorescence intensity (FU/ODintact) (
) and percentage of permeabilized cells (
) in the FL3-A channel for Re2133/pCB1 with kanamycin: with fructose pulse (a) and continuous feeding (b). Boxplot comparison on fluorescence intensity distribution in the FL1-A channel for Re2133/pCB1 with kanamycin: with fructose pulse (c) and continuous feeding (d). Evolution of the decimal reduction rate for Re2133/pCB1 with kanamycin: with fructose pulses (e) and continuous feeding (f) based on plate count (□) and flow cytometry (●) measurements. Black vertical lines represent the beginning of the fed-batch phase and orange vertical lines represent fructose pulses. Purple arrows represent additions (kanamycin, 100 mg·L−1; elements and phosphorus solutions)