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Table 2 Strains and plasmids used in this study

From: Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium

Strain or plasmid

Description/characteristic

Source/reference

Strain

  

 Escherichia coli DH5α

Cloning strain

TSINGKE (China, Beijing)

 Escherichia coli S17-1

A vector donor in conjugation, integrates RP4 derivative in chromosome

Simon (1984)

 S17_sqr

Derivate of S17-1, containing plasmid pBBR-ptac-sqr

This study

 Halomonas salifodinae IM328

Wild type, isolated from a soda-saline lake in China

This study

 H. salifodinae IM328_sqr

Derivate of IM328, containing plasmid pBBR-ptac-sqr

This study

 H. salifodinae IM328_ck

Derivate of IM328, containing plasmid pBBR-ptac-ck

This study

Plasmid

  

 pBBR-ptac-rfp

Derivate of plasmid pBBR1MCS, tac promoter, expressing rfp gene

Kovach et al. (1995), Mu et al. (2017)

 pBBR-ptac-sqr

Derivate of plasmid pBBR1MCS, tac promoter, expressing sqr from Spiribacter sp. IM2438

This study

 pBBR-ptac-ck

rfp deletion from pBBR-ptac-rfp vector

This study