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Fig. 6 | AMB Express

Fig. 6

From: Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium

Fig. 6

Sulfide oxidation by H. salifodinae IM328_sqr. The experiments were carried out in the 1.5 mL centrifuge tube. Na2S was added to 1000 µM to initiate the reaction. Detection and calculation of the sulfide contents consumed in the reaction system were done after 5 min. a Comparison of the desulfurizing activity of IM328_sqr and IM328_ck under aerobic conditions. Sulfide oxidation by live and heated killed cells. Cells were harvested from LB-60, washed and resuspended in 1 mL of 25 mM tricine buffer (pH 8.0) at 37 °C, 200 rpm. The biomass was equivalent for H. salifodinae IM328_sqr and IM328_ck (OD600 of approximate 4). b Optimization of the pH value of the whole-cell catalysis of IM328_sqr. Cells were resuspended at OD600 of 2 in 25 mM buffer corresponding to different pH values, respectively. Averages (n = 3) with standard deviations (error bar) are shown

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