Differential induction of antioxidant and anti-inflammatory phytochemicals in agitated micro-shoot cultures of Ajuga integrifolia Buch. Ham. ex D.Don with biotic elicitors

Ullah, Muhammad Asad; Gul, Faiza Zareen; Khan, Taimoor; Bajwa, Muhammad Naeem; Drouet, Samantha; Tungmunnithum, Duangjai; Giglioli-Guivarc’h, Nathalie; Liu, Chunzhao; Hano, Christophe; Abbasi, Bilal Haider

doi:10.1186/s13568-021-01297-3

AMB Express

Table 1 HPLC analysis of phytochemical compounds in agitated micro-shoot cultures of Ajuga integrifolia

From: Differential induction of antioxidant and anti-inflammatory phytochemicals in agitated micro-shoot cultures of Ajuga integrifolia Buch. Ham. ex D.Don with biotic elicitors

Treatment	Conc	Phytochemicals quantification via HPLC (in µg/g DW)
Treatment	Conc	Chlorogenic Acid	Caffeic Acid	Rosmarinic Acid	Apigenin	Luteolin	Quercetin	Harpagide	Aucubin	Harpagoside	Catalpol	8-O-acetyl-harpagoside
Control	–	165.11 ± 2.48	200.17 ± 1.16	386.41 ± 4.67	483.47 ± 3.55	483.47 ± 2.31	501.02 ± 4.66	303.58 ± 5.33	166.03 ± 2.96	147.69 ± 4.46	101.85 ± 2.55	211.88 ± 3.21
Yeast extract (mg/L)	1	187.70 ± 5.34*	232.82 ± 4.25***	428.33 ± 3.46***	508.52 ± 2.34*	474.10 ± 6.26	508.52 ± 2.71	292.13 ± 3.17	155.65 ± 4.34	137.45 ± 2.70*	101.06 ± 2.41	192.04 ± 2.34*
	10	214.59 ± 2.16***	256.47 ± 2.84***	494.51 ± 3.54***	504.01 ± 4.61*	486.35 ± 2.58	539.32 ± 4.93*	253.01 ± 1.92**	141.00 ± 2.62*	122.34 ± 3.92**	94.34 ± 3.07	178.34 ± 4.58**
	50	182.31 ± 4.79**	225.53 ± 1.76***	413.55 ± 4.24***	459.56 ± 6.52*	494.18 ± 4.96	494.18 ± 3.09	293.83 ± 4.84*	165.71 ± 3.75	147.41 ± 4.23	110.80 ± 6.48	202.32 ± 4.67
	100	180.50 ± 6.73**	210.82 ± 1.95**	410.03 ± 5.16***	477.80 ± 3.86	477.80 ± 3.71	495.15 ± 1.82	335.01 ± 2.76**	186.56 ± 2.98**	168.00 ± 1.45**	121.61 ± 2.64**	242.23 ± 2.49**
	200	213.40 ± 3.46***	252.45 ± 4.84***	488.93 ± 2.58***	513.50 ± 5.94*	496.12 ± 1.02	513.50 ± 2.46*	249.05 ± 6.52***	138.80 ± 2.41**	120.42 ± 5.63**	92.86 ± 1.77	175.55 ± 2.85**
Pectin (mg/L)	1	281.72 ± 4.75 ***	359.52 ± 3.73 ***	668.56 ± 1.52 ***	563.43 ± 2.85 ***	546.12 ± 1.07 **	580.74 ± 5.57 ***	174.86 ± 3.06 ***	101.65 ± 4.56 ***	92.50 ± 2.50 ***	65.04 ± 3.28 **	129.10 ± 1.72 ***
	10	254.48 ± 3.52***	312.04 ± 2.91***	593.47 ± 2.34***	521.69 ± 2.52**	504.61 ± 2.81**	538.77 ± 2.84**	199.59 ± 1.46***	118.34 ± 1.04***	100.28 ± 6.17**	73.19 ± 4.64*	136.39 ± 2.93***
	50	294.12 ± 0.24***	343.72 ± 6.84***	680.20 ± 4.19***	579.61 ± 4.46***	545.06 ± 1.46**	596.89 ± 4.92***	174.52 ± 2.40***	101.45 ± 2.84***	83.18 ± 3.84***	64.91 ± 5.97**	128.85 ± 5.46**
	100	192.93 ± 1.13***	228.82 ± 4.25**	425.21 ± 3.73***	499.81 ± 6.01	449.06 ± 4.07**	499.81 ± 6.03	269.23 ± 2.08***	152.98 ± 3.53*	135.10 ± 2.14*	99.32 ± 2.23	188.75 ± 2.57*
	250	202.76 ± 3.36***	255.45 ± 3.59***	455.23 ± 5.84***	537.19 ± 7.16**	484.43 ± 2.72	519.60 ± 3.14*	270.60 ± 4.79***	149.74 ± 3.26*	131.15 ± 1.57*	93.96 ± 4.52*	186.93 ± 4.14*
	500	240.56 ± 2.12***	297.75 ± 2.64***	557.32 ± 2.78***	520.63 ± 3.89**	520.63 ± 3.18**	538.25 ± 1.05**	224.56 ± 1.43***	122.09 ± 4.74***	112.78 ± 2.92**	84.83 ± 2.44**	150.04 ± 3.64**

Values represent means ± standard errors from triplicates; *p < 0.05, **p < 0.01, ***p < 0.001 compared to control

Back to article page