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Table 1 HPLC analysis of phytochemical compounds in agitated micro-shoot cultures of Ajuga integrifolia

From: Differential induction of antioxidant and anti-inflammatory phytochemicals in agitated micro-shoot cultures of Ajuga integrifolia Buch. Ham. ex D.Don with biotic elicitors

Treatment Conc Phytochemicals quantification via HPLC (in µg/g DW)
Chlorogenic Acid Caffeic Acid Rosmarinic Acid Apigenin Luteolin Quercetin Harpagide Aucubin Harpagoside Catalpol 8-O-acetyl-harpagoside
Control 165.11 ± 2.48 200.17 ± 1.16 386.41 ± 4.67 483.47 ± 3.55 483.47 ± 2.31 501.02 ± 4.66 303.58 ± 5.33 166.03 ± 2.96 147.69 ± 4.46 101.85 ± 2.55 211.88 ± 3.21
Yeast extract (mg/L) 1 187.70 ± 5.34* 232.82 ± 4.25*** 428.33 ± 3.46*** 508.52 ± 2.34* 474.10 ± 6.26 508.52 ± 2.71 292.13 ± 3.17 155.65 ± 4.34 137.45 ± 2.70* 101.06 ± 2.41 192.04 ± 2.34*
10 214.59 ± 2.16*** 256.47 ± 2.84*** 494.51 ± 3.54*** 504.01 ± 4.61* 486.35 ± 2.58 539.32 ± 4.93* 253.01 ± 1.92** 141.00 ± 2.62* 122.34 ± 3.92** 94.34 ± 3.07 178.34 ± 4.58**
50 182.31 ± 4.79** 225.53 ± 1.76*** 413.55 ± 4.24*** 459.56 ± 6.52* 494.18 ± 4.96 494.18 ± 3.09 293.83 ± 4.84* 165.71 ± 3.75 147.41 ± 4.23 110.80 ± 6.48 202.32 ± 4.67
100 180.50 ± 6.73** 210.82 ± 1.95** 410.03 ± 5.16*** 477.80 ± 3.86 477.80 ± 3.71 495.15 ± 1.82 335.01 ± 2.76** 186.56 ± 2.98** 168.00 ± 1.45** 121.61 ± 2.64** 242.23 ± 2.49**
200 213.40 ± 3.46*** 252.45 ± 4.84*** 488.93 ± 2.58*** 513.50 ± 5.94* 496.12 ± 1.02 513.50 ± 2.46* 249.05 ± 6.52*** 138.80 ± 2.41** 120.42 ± 5.63** 92.86 ± 1.77 175.55 ± 2.85**
Pectin (mg/L) 1 281.72 ± 4.75 *** 359.52 ± 3.73 *** 668.56 ± 1.52 *** 563.43 ± 2.85 *** 546.12 ± 1.07 ** 580.74 ± 5.57 *** 174.86 ± 3.06 *** 101.65 ± 4.56 *** 92.50 ± 2.50 *** 65.04 ± 3.28 ** 129.10 ± 1.72 ***
10 254.48 ± 3.52*** 312.04 ± 2.91*** 593.47 ± 2.34*** 521.69 ± 2.52** 504.61 ± 2.81** 538.77 ± 2.84** 199.59 ± 1.46*** 118.34 ± 1.04*** 100.28 ± 6.17** 73.19 ± 4.64* 136.39 ± 2.93***
50 294.12 ± 0.24*** 343.72 ± 6.84*** 680.20 ± 4.19*** 579.61 ± 4.46*** 545.06 ± 1.46** 596.89 ± 4.92*** 174.52 ± 2.40*** 101.45 ± 2.84*** 83.18 ± 3.84*** 64.91 ± 5.97** 128.85 ± 5.46**
100 192.93 ± 1.13*** 228.82 ± 4.25** 425.21 ± 3.73*** 499.81 ± 6.01 449.06 ± 4.07** 499.81 ± 6.03 269.23 ± 2.08*** 152.98 ± 3.53* 135.10 ± 2.14* 99.32 ± 2.23 188.75 ± 2.57*
250 202.76 ± 3.36*** 255.45 ± 3.59*** 455.23 ± 5.84*** 537.19 ± 7.16** 484.43 ± 2.72 519.60 ± 3.14* 270.60 ± 4.79*** 149.74 ± 3.26* 131.15 ± 1.57* 93.96 ± 4.52* 186.93 ± 4.14*
500 240.56 ± 2.12*** 297.75 ± 2.64*** 557.32 ± 2.78*** 520.63 ± 3.89** 520.63 ± 3.18** 538.25 ± 1.05** 224.56 ± 1.43*** 122.09 ± 4.74*** 112.78 ± 2.92** 84.83 ± 2.44** 150.04 ± 3.64**
  1. Values represent means ± standard errors from triplicates; *p < 0.05, **p < 0.01, ***p < 0.001 compared to control