Table 2 Growth comparison of Acetobacter sp. CIP 58.66 with glycerol as substrate, at different initial pH
Shake flask culturesa | Biocatalyst productionb | ||||
|---|---|---|---|---|---|
Initial pH | 4.0 | 4.5 | 5.0 | 6.5 | 5.0 |
DW (gDW L−1) | 0.14 ± 0.01 | 0.33 ± 0.04 | 1.14 ± 0.04 | 0.43 ± 0.02 | 0.88 ± 0.05 |
Glycerol consumption (%)c | 1.0 ± 0.8 | 4.6 ± 1.9 | 16.9 ± 0.7 | 5.8 ± 0.6 | 18.6 ± 1.7 |
Biomass yield (\({\text{g}}_{\text{DW}}\, {\text{g}}_{\text{glycerol}}^{-1}\)) | NDd | 0.83 ± 0.28 | 0.69 ± 0.05 | 0.76 ± 0.06 | 0.40 ± 0.05 |
Final pH | 4.10 ± 0.02 | 4.58 ± 0.08 | 5.38 ± 0.03 | 6.55 ± 0.03 | 6.94 ± 0.13 |
µmax,1 (h−1) | 0.10 ± 0.01 | 0.15 ± 0.004 | 0.18 ± 0.004 | 0.11 ± 0.004 | 0.24 ± 0.02 |
R2 | 0.94 | 0.98 | 0.99 | 0.98 | 0.88 |
µmax,2 (h−1) | 0.03 ± 0.002 | 0.06 ± 0.002 | 0.09 ± 0.002 | 0.07 ± 0.001 | 0.18 ± 0.01 |
R2 | 0.92 | 0.97 | 0.99 | 0.99 | 0.96 |