Fig. 3

Growth of Acetobacter sp. CIP 58.66 and glycerol consumption during the sequential process. 1. Primary, batch growth on glycerol; this step is performed in the same conditions for all experiments. 2. Secondary growth during 1,3-PDO fed-batch bioconversion, at different pH (4.0, 4.5 or 5.0). For each growth phase, logarithmic growth is calculated on the basis of the initial DW of the concerned phase. For each pH condition, n = 2. The initial working volume was 1.02 L, and the final estimated volumes varied between 0.83 and 0.91 L, depending on replicates