Fig. 3
Effect of volume
ratio (VR, 0.38 and 3.50) and tie-line length (TLL) on a hyaluronic acid (HA) (□) and
protein (
) recovery in the bottom phase of polyethylene glycol (PEG)
8000–citrate aqueous two-phase systems (ATPS); and on b HA purity (TLL 24 (
), 36 (
), and 43 (
) % w/w). The crude
extract (molecular weight: 3 MDa) was loaded to the ATPS at a final
concentration of 10% (w/w). Bars represent the sample mean ± SEM
of experimental triplicates. Bars within the same graph that do not share the
same uppercase (HA) or lowercase (protein) letter are significantly different (p < 0.05)