Fig. 2
Effect of volume
ratio (VR, 0.38, 1.00, and 3.50) and tie-line length (TLL, 24 (
),
36 (
), and 43 (
) % w/w) on hyaluronic acid (HA) recovery in the bottom
phase of polyethylene glycol (PEG) 8000–citrate aqueous two-phase systems (ATPS).
Two pure HA solutions of different molecular weights: 1–2 MDa (HA1.5) and 3–4 MDa (HA3.5) were evaluated. HA solutions were loaded to the ATPS at a final
concentration of 10% (w/w). Bars represent the sample mean ± SEM
of experimental triplicates. Bars within the same graph that do not share the
same uppercase letter are
significantly different (p < 0.05)