Antibiotic resistance, virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains recovered from clinical samples

AMB Express

Table 1 The primer sequences of target genes which used in this study

Target gene	Primer sequence (5ʹ to 3ʹ)	Annealing temperature (°C)	References
acrAB	F ATCAGCGGCCGGATTGGTAAA R CGGGTTCGGGAAAATAGCGCG	53	Wasfi et al. (2016)
tolC	F ATCAGCAACCCCGATCTGCGT R CCGGTGACTTGACGCAGTCCT	51
mdtk	F GCGCTTAACTTCAGCTCA R GATGATAAATCCACACCAGAA	43
iucC	F TGGATTGATGCTCAAACTCTG R TGCATCGCTCATTGACAGTA		Wasfi et al. (2016)
mrkA	F ACGTCTCTAACTGCCAGGC R TAGCCCTGTTGTTTGCTGGT	55	Vuotto et al. (2017)
mrkD	F CCACCAACTATTCCCTCGAA R ATGGAACCCACATCGACATT	43	Sahly et al. (2008), El Fertas-Aissani et al. (2013)
fimH	F GCCAACGTCTACGTTAACCTG R ATATTTCACGGTGCCTGAAAA	43	Wasfi et al. (2016)
entB	F CTGCTGGGAAAAGCGATTGTC R AAGGCGACTCAGGAGTGGCTT	49	Wasfi et al. (2016)
traT	F GGTGTGGTGCGATGAGCACAG R CACGGTTCAGCCATCCCTGAG	55	El Fertas-Aissani et al. (2013)
rmpA	F ACTGGGCTACCTCTGCTTCA R CTTGCATGAGCCATCTTTCA	53	Siu et al. (2011)
ybtS magA	F GACGGAAACAGCACGGTAAA R GAGCATAATAAGGCGAAAGA F GGTGCTCTTTACATCATTGC R GCAATGGCCATTTGCGTTAG	60	Compain et al. (2014)
htrA	F AGAGTTCGCCGTTTTGCCAGGG R ATCAGAGCGCGGATCTTTGCCG	60	Rasheed et al. (2016)
16 S rRNA	F AGCCGACCTGAGAGGGTGA R TCTGGACCGTGTCTCAGTTCC	55	Marroquin et al. (2019)