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Fig. 2 | AMB Express

Fig. 2

From: Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3

Fig. 2

Expression of Shh, Gli1, Smo and PHC3 in CD133+ and in CD133 lung cancer cells. a, b QPCR data shows that the mRNA levels of Shh, Gli1, Smo and PHC3 in A549/CD133+ and H460/CD133+ are higher than those in A549/CD133 and H460/CD133. ce Western blotting data shows that the protein levels of Shh, Gli1, Smo and PHC3 in A549/CD133+ and H460/CD133+ cells are higher than those in A549/CD133 and H460/CD133 cells. The relative protein levels were quantified with Image J software. N = 3, Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001

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