Fig. 2

Expression of Shh, Gli1, Smo and PHC3 in CD133⁺ and in CD133⁻ lung cancer cells. a, b QPCR data shows that the mRNA levels of Shh, Gli1, Smo and PHC3 in A549/CD133⁺ and H460/CD133⁺ are higher than those in A549/CD133⁻ and H460/CD133⁻. c–e Western blotting data shows that the protein levels of Shh, Gli1, Smo and PHC3 in A549/CD133⁺ and H460/CD133⁺ cells are higher than those in A549/CD133⁻ and H460/CD133⁻ cells. The relative protein levels were quantified with Image J software. N = 3, Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001