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Table 5 The effects of various surfactants/NADESs on cell membrane permeability

From: Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

Additives

OD260

OD280

PBS buffer

0.247 ± 0.02

0.205 ± 0.01

Tween-20

0.329 ± 0.02

0.276 ± 0.03

Tween-80

0.273 ± 0.04

0.235 ± 0.01

Span-20

0.266 ± 0.02

0.229 ± 0.04

Span-60

0.282 ± 0.04

0.232 ± 0.01

Span-80

0.295 ± 0.04

0.221 ± 0.02

ChCl:IPA (1:1)

0.247 ± 0.03

0.228 ± 0.03

ChCl:Gly (1:1)

0.302 ± 0.02

0.264 ± 0.04

ChCl:U (1:1)

0.269 ± 0.02

0.233 ± 0.04

ChCl:EG (1:1)

0.309 ± 0.04

0.271 ± 0.01

ChCl:Lys (1:1)

0.291 ± 0.03

0.262 ± 0.03

Tween-20 & ChCl:U (1:1)

0.294 ± 0.03

0.243 ± 0.04

Tween-20 & ChCl:Lys (1:1)

0.309 ± 0.02

0.257 ± 0.02

Tween-20 & ChCl:EG (1:1)

0.388 ± 0.03

0.291 ± 0.04

  1. Reaction conditions: 17.2 g (DCW)/L recombinant E. coli cells, 0.6% (w/v) various surfactants, 4% (w/v) various NADESs, PBS buffer (pH 7.0), 30 ℃, 200 rpm for 18 h