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Table 5 The effects of various surfactants/NADESs on cell membrane permeability

From: Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

Additives OD260 OD280
PBS buffer 0.247 ± 0.02 0.205 ± 0.01
Tween-20 0.329 ± 0.02 0.276 ± 0.03
Tween-80 0.273 ± 0.04 0.235 ± 0.01
Span-20 0.266 ± 0.02 0.229 ± 0.04
Span-60 0.282 ± 0.04 0.232 ± 0.01
Span-80 0.295 ± 0.04 0.221 ± 0.02
ChCl:IPA (1:1) 0.247 ± 0.03 0.228 ± 0.03
ChCl:Gly (1:1) 0.302 ± 0.02 0.264 ± 0.04
ChCl:U (1:1) 0.269 ± 0.02 0.233 ± 0.04
ChCl:EG (1:1) 0.309 ± 0.04 0.271 ± 0.01
ChCl:Lys (1:1) 0.291 ± 0.03 0.262 ± 0.03
Tween-20 & ChCl:U (1:1) 0.294 ± 0.03 0.243 ± 0.04
Tween-20 & ChCl:Lys (1:1) 0.309 ± 0.02 0.257 ± 0.02
Tween-20 & ChCl:EG (1:1) 0.388 ± 0.03 0.291 ± 0.04
  1. Reaction conditions: 17.2 g (DCW)/L recombinant E. coli cells, 0.6% (w/v) various surfactants, 4% (w/v) various NADESs, PBS buffer (pH 7.0), 30 ℃, 200 rpm for 18 h