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Table 3 Redox specific activities of cell-free extract on different additives and NAD+

From: Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

Isopropanola

Tween-20b

ChCl:Lysc

NAD+

Specific activity (U/mg)

ND

+ 

ND

+ 

ND

+ 

+ 

2.21 ± 0.04

+ 

+ 

ND

+ 

ND

+ 

+ 

1.73 ± 0.04

+ 

+ 

+ 

3.07 ± 0.09

  1. Reaction conditions: 1.0 mL phosphate buffer (pH 7.5), 1.0 mL cell-free extract (5.25 mg of total protein), with or without various additives, 0.3 mM NAD+
  2. “ + ” means added the additives; “−”means not added; “ND” means not detected
  3. a15% (v/v) isopropanol
  4. b0.6% (w/v) Tween-20
  5. c4% (w/v) ChCl:Lys (1:1)