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Table 3 Redox specific activities of cell-free extract on different additives and NAD+

From: Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

Isopropanola Tween-20b ChCl:Lysc NAD+ Specific activity (U/mg)
− − − − ND
− − − +  ND
+  − − − ND
+  − − +  2.21 ± 0.04
− +  − +  ND
− − +  − ND
− − +  +  1.73 ± 0.04
+  − +  +  3.07 ± 0.09
  1. Reaction conditions: 1.0 mL phosphate buffer (pH 7.5), 1.0 mL cell-free extract (5.25 mg of total protein), with or without various additives, 0.3 mM NAD+
  2. “ + ” means added the additives; “−”means not added; “ND” means not detected
  3. a15% (v/v) isopropanol
  4. b0.6% (w/v) Tween-20
  5. c4% (w/v) ChCl:Lys (1:1)
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