Isopropanola | Tween-20b | ChCl:Lysc | NAD+ | Specific activity (U/mg) |
---|
− | − | − | − | ND |
− | − | − | + | ND |
+ | − | − | − | ND |
+ | − | − | + | 2.21 ± 0.04 |
− | + | − | + | ND |
− | − | + | − | ND |
− | − | + | + | 1.73 ± 0.04 |
+ | − | + | + | 3.07 ± 0.09 |
- Reaction conditions: 1.0 mL phosphate buffer (pH 7.5), 1.0 mL cell-free extract (5.25 mg of total protein), with or without various additives, 0.3 mM NAD+
- “ + ” means added the additives; “−”means not added; “ND” means not detected
- a15% (v/v) isopropanol
- b0.6% (w/v) Tween-20
- c4% (w/v) ChCl:Lys (1:1)