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Fig. 5 | AMB Express

Fig. 5

From: Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

Fig. 5

Effects of key reaction parameters on the (R)-MTF-PEL production with recombinant E. coli cells in a Tween-20/ChCl:Lys-containing system. Reaction conditions: a Effect of initial buffer pH: 200 mM 3'-(trifluoromethyl)acetophenone, 15% (v/v) isopropanol, 0.6% (w/v) Tween-20, 4% (w/v) ChCl:Lys, 17.2 g (DCW)/L recombinant E. coli cells, 30 ℃, 200 rpm, 21 h. b Impact of the ratio of biocatalyst/substrate: 200 mM 3'-(trifluoromethyl)acetophenone, 15% (v/v) isopropanol, 0.6% (w/v) Tween-20, 4% (w/v) ChCl:Lys, PBS buffer (pH 7.0), 30 ℃, 200 rpm, 21 h. c Time course of (R)-MTF-PEL production under 200 mM and 400 mM substrate concentration: 15% (v/v) isopropanol, 0.6% (w/v) Tween-20, 4% (w/v) ChCl:Lys, PBS buffer (pH 7.0), 30 ℃, 200 rpm, 21 h. 12.6 g (DCW)/L E. coli cells under 200 mM substrate concentration (Black star), 25.2 g (DCW)/L recombinant E. coli cells under 400 mM substrate concentration (Black circle)

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