Fig. 2
Transformation of Synechocystis strains. All transgenes used in the study were expressed under the control of the psbA2 promoter (Prm) and 5ST1T2 terminator (Trm). Intergenic regions (IR) present between individual genes in the gene sets are represented as grey boxes. Numbered letters and arrows represent the primer pairs, and the direction of the amplifications, respectively. a Genes encoding the arabinose transporters were inserted into neutral site 1 in the Synechocystis WT genome using upstream (N1UP) and downstream (N1DW) regions for homologous recombination. The kanamycin resistance cassette (Kmr) was used for the selection and segregation of the transformants. b The araBAD gene set, encoding enzymes that funnel arabinose into the pentose phosphate pathway, was inserted into neutral site 2 in the genomes of Synechocystis strains carrying one of the three arabinose transporter genes and the WT strain using upstream (N2UP) and downstream (N2DW) regions for homologous recombination. The erythromycin resistance cassette (Emr) was used for the selection and segregation of the transformants