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Fig. 5 | AMB Express

Fig. 5

From: Production of R- and S-1,2-propanediol in engineered Lactococcus lactis

Fig. 5

Production of 1,2-PDO in L. lactis LL1 from 5% mannitol and 5% gluconate in a jar fermenter controlled with a pH–stat. L. lactis LL1 was cultivated for 96 h at 30 °C in M17 medium supplemented with 5% mannitol and 5% gluconate under microaerobic conditions. The medium was supplemented with 0.3 g/L acetate. The pH of the culture was maintained at 6.8 by addition of 2 M NaOH during cultivation. Closed triangles, cell growth (OD600); closed circles, S-1,2-PDO; shaded diamonds, L-lactate; open triangles, mannitol; open squares, gluconate; open diamonds, concentration of DO in the culture; open circles, pH of the culture. This experiment was performed in duplicate, and the average is represented with error bars

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