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Fig. 4 | AMB Express

Fig. 4

From: Production of R- and S-1,2-propanediol in engineered Lactococcus lactis

Fig. 4

Production of 1,2-PDO in L. lactis LL1 with 1% gluconate and 1% mannitol in flask cultivation. L. lactis LL1 was cultivated for 72 h at 30 °C in M17 medium supplemented with 1% mannitol and 1% gluconate under microaerobic conditions. The medium was supplemented with 0.3 g/L acetate. The initial pH of the culture was 7.0. The culture was supplemented with 1 M MOPS (pH 7.0) every 24 h so that the pH of the culture was readjusted to 7.0. Closed triangles, cell growth (OD600); closed circles, S-1,2-PDO; shaded diamonds, L-lactate; open triangles, mannitol; open squares, gluconate. This experiment was performed in duplicate, and the average is represented with error bars

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