Fig. 1

Staining of P. denitrificans cells with different fluorescent dyes. In all cases, cells were grown to an OD₆₆₀ of about 0.75–1 and stained with: A MitoTracker™ Green FM (MTG) (1 µM); B FM4-64 (1 µM); C Nile Red (1 µM); D Double staining with MTG (1 µM) and Syto80 (500 nM). E–G Details of daughter cells from a recently divided mother cell. MTG image (E), Syto80 image (F), overlay (G). Cells were imaged using two different lasers (a blue-argon Laser, 488 nm, for excitation of MTG and a green-helium/neon laser, 543 nm, for excitation of Syto80)