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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Rapid diagnostics for Gnomoniopsis smithogilvyi (syn. Gnomoniopsis castaneae) in chestnut nuts: new challenges by using LAMP and real-time PCR methods

Fig. 1

Comparison of sensitivity among qPCR (left), P-LAMP (middle), V-LAMP (right) assays using ten 1:5 serial dilutions ranged between 10 ng and 5.12 fg/µL of reference Gnomoniopsis smithogilvyi DNA (strain CBS 142044). The assessment for the V-LAMP assay was based on the HNB color change visualization of the LAMP products (Positive samples = blue color change in reaction, negative samples = violet color reaction) and based on gel electrophoresis: L-1000 bp ladder, Dilutions A-G showed positive reactions while no amplification was observed in dilution H and NTC (No Template Control)

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