Fig. 5
Effect of n-hexane on the promoter activity of katE, katG, sodA, or sodB. A 100-μl culture of the overnight-grown strain BW25113(pMCkatEp) (A), BW25113(pMCkatGp) (B), BW25113(pMCsodAp) (C), or BW25113(pMCsodBp) (D) was inoculated into 10 ml of fresh LBGMg liquid medium containing 50 μg/ml ampicillin without or with 1 ml of n-hexane. The culture was incubated at 37 °C until reaching an OD600 of approximately 0.6. Cells were treated with chloroform and assayed for β-galactosidase activity. Values indicate the means and standard deviations of the results from three independent experiments