Fig. 4From: Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coliLevels of intracellular ROS in E. coli cells measured by using carboxy-H2DCFDA. ROS levels were measured in the strains exposed to TBHP (tert-butyl hydroperoxide), n-hexane or cyclohexane as described in the Methods. The relative specific fluorescence shows the ratio of the specific fluorescence in each strain divided by that in strain BW25113 not exposed to the organic solvent. Abbreviations: BW, BW25113; ∆katE∆katG, BW25113∆katE∆katG; ∆sodA∆sodB, BW25113∆sodA∆sodB. Mean values and standard deviations for three independent experiments are shownBack to article page