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Table 1 The primers of key enzyme genes

From: Effects of carbon concentration, oxygen, and controlled pH on the engineering strain Lactiplantibacillus casei E1 in the production of bioethanol from sugarcane molasses

Target

Primer

Sequence

GK

Forward

5′ ATTGAGGTGTAATAGGTCGGTGG 3′

Reverse

5′ CGATTTTATGACGATTGATGCC 3′

INV

Forward

5′ AGACGCAGACTTGTTGTTTCCC 3′

Reverse

5′ GACGTTAGATGATGGCGATGAG 3′

PFK

Forward

5′ CATTGCCAAAGAAGCGACC 3′

Reverse

5′ AAGACAACGATTCATCTGCCTG 3′