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Table 4 Apparent kinetic parameters of ∆22Bt3590

From: A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides

Substrates

GlcNAcαMU (1)

GlcNAcα1–4GlcAβProNHFmoc (2)

KM (mM)

(4.6 ± 0.4) × 10−3

2.19 ± 0.16

kcat (s−1)

3.68 ± 0.07

3.57 ± 0.09

kcat/KM (s−1 mM−1)

8.0 × 102

1.63