From: A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides
Substrates
GlcNAcαMU (1)
GlcNAcα1–4GlcAβProNHFmoc (2)
KM (mM)
(4.6 ± 0.4) × 10−3
2.19 ± 0.16
kcat (s−1)
3.68 ± 0.07
3.57 ± 0.09
kcat/KM (s−1 mM−1)
8.0 × 102
1.63