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Table 4 Apparent kinetic parameters of ∆22Bt3590

From: A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides

Substrates GlcNAcαMU (1) GlcNAcα1–4GlcAβProNHFmoc (2)
KM (mM) (4.6 ± 0.4) × 10−3 2.19 ± 0.16
kcat (s−1) 3.68 ± 0.07 3.57 ± 0.09
kcat/KM (s−1 mM−1) 8.0 × 102 1.63