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Table 1 Primers used for cloning full-length and truncated bacterial α-N-acetylglucosaminidases

From: A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides

Primers Oligonucleotidesa
CpGH89 Forward 5′TTGGCTCTCGAGGGTGTTGAAATTACGGAAGGGGTTACTGTAACTGC3′
Reverse 5′AGCCAAGGATCCTTATGATTCATTTTCACCTAATATTTTATCCATATTAGTTACTGAATAACTTTCCATGGC3′
tCpGH89 Forward 5′CATTCAAAAATAGTTTATGATAAGAGTGAATTTGAA AAAGCTATTGAAATATTTGC3′
Reverse 5′TATTCCAAAGCCTGGTCTTGCATTTATAATAGACTCAGC3′
Bf0576 Forward 5′GTGTGTCTCGAGATGAATCGTAAATCAATACT3′
∆17Bf0576 Forward 5′GTGTGTCTCGAGGCAATGGCTTCTCCGGTAAC3′
Bf0576 Reverse 5′GTGTGTGGATCCTTATTCAACCGCTTGCATCA3′
Bt0438 Forward 5′GTGTGTCTCGAGATGAACAGACAATACTTCTA3′
∆24Bt0438 Forward 5′GTGTGTCTCGAGAGTAACCCAGTATTAGAACA3′
Bt0438 Reverse 5′GTGTGTGGATCCTTAATAAAAATATTGCATATATT3′
Bt3590 Forward 5′GTGTGTCTCGAGATGAATCATAAATACCTATA3′
∆22Bt3590 Forward 5′GTGTGTCTCGAGACAGGCCCTCCTGTATTAAA3′
Bt3590 Reverse 5′GTGTGTGGATCCTTATTGTGCTTTGGTAAAGT3′
  1. aRestriction sites are italicized and underlined