A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides

AMB Express

Table 1 Primers used for cloning full-length and truncated bacterial α-N-acetylglucosaminidases

Primers	Oligonucleotides^a
CpGH89	Forward	5′TTGGCTCTCGAGGGTGTTGAAATTACGGAAGGGGTTACTGTAACTGC3′
CpGH89	Reverse	5′AGCCAAGGATCCTTATGATTCATTTTCACCTAATATTTTATCCATATTAGTTACTGAATAACTTTCCATGGC3′
tCpGH89	Forward	5′CATTCAAAAATAGTTTATGATAAGAGTGAATTTGAA AAAGCTATTGAAATATTTGC3′
tCpGH89	Reverse	5′TATTCCAAAGCCTGGTCTTGCATTTATAATAGACTCAGC3′
Bf0576	Forward	5′GTGTGTCTCGAGATGAATCGTAAATCAATACT3′
∆17Bf0576	Forward	5′GTGTGTCTCGAGGCAATGGCTTCTCCGGTAAC3′
Bf0576	Reverse	5′GTGTGTGGATCCTTATTCAACCGCTTGCATCA3′
Bt0438	Forward	5′GTGTGTCTCGAGATGAACAGACAATACTTCTA3′
∆24Bt0438	Forward	5′GTGTGTCTCGAGAGTAACCCAGTATTAGAACA3′
Bt0438	Reverse	5′GTGTGTGGATCCTTAATAAAAATATTGCATATATT3′
Bt3590	Forward	5′GTGTGTCTCGAGATGAATCATAAATACCTATA3′
∆22Bt3590	Forward	5′GTGTGTCTCGAGACAGGCCCTCCTGTATTAAA3′
Bt3590	Reverse	5′GTGTGTGGATCCTTATTGTGCTTTGGTAAAGT3′