Fig. 7

Substrate specificities of ∆22Bt3590 at 37 °C (A) or 30 °C (B) using GlcNAcαMU (1) and synthetic α-GlcNAc-terminated fluorescent-tagged heparosan oligosaccharides of varied lengths (2–6, Fig. 4) as potential substrates. Reaction times vary from 20 min (white columns), 1 h (patten filled columns), 4 h (black columns), to 24 h (gray columns)