Skip to main content
AMB Express
Fig. 3 | AMB Express

Fig. 3

From: A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides

Fig. 3

SDS-PAGE analysis of the expression and purification of recombinant bacterial α-N-acetylglucosaminidases including His6-CpGH89, and loop-deletion CpGH89 (His6-tCpGH89), His6-∆17Bf0576, His6-∆24Bt0438, His6-∆22Bt3590. Lanes: M, Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder (10–250 kDa) for His6-∆22Bt3590 samples and Thermo Scientific™ PageRuler™ Prestained Protein Ladder (10–180 kDa) for other samples. The size of the marker with an asterisk on the left is 100 kDa and the size of the marker with a diamond on the left is 75 kDa); BI, whole cell extract before induction; AI, whole cell extract after induction; L, lysate after induction; P, Ni2+-NTA column purified protein

Back to article page