Table 4 Substrate specificity and kinetic parameters of Lcc120-3
Substrate | Lcc120-3 (Mic-mg-MCO) | |
|---|---|---|
Activity (U/mg protein) | Km (mM)/Vmax (U/mg protein)/kcat (s−1) | |
DMP | 2.0 | 10.5/2.3/2.3 |
o-Guaiacol | 0.05 | – |
o-Catechol | 2.47 | – |
Pyrogallol | 13.9 | – |
Gallic acid | 0.64 POU | – |
ABTS | 6.63 | – |
Syringaldazine | N.D | – |
Bilirubin | N.D | – |
l-Tyrosine | N.D | – |
l-DOPA (l-3,4-Dihydroxyphenylalanine) | N.D | – |
Iodide | N.D | – |