Table 1 PCR primers for isolating the core genes of MCO from metagenomes
Primer | Sequence (5′-3′) |
|---|---|
BacBOXdgFw1 | ACNTGGGGNTAYAAYGGNWS |
BacBOXdgFw2 | ACNTGGGGNGTNAAYGGNWS |
BacBOXdgRv1 | CATCATNCCNYYRTCYTCRTG |
BacBOXdgRv2 | ADRTGRCARTGRTACATRWA |
BacBOXdgFw1GAM | CTGCCGCCGACCCGT ACNTGGGGNTAYAAYGGNWS |
BacBOXdgFw2GAM | CTGCCGCCGACCCGT ACNTGGGGNGTNAAYGGNWS |
BacBOXdgRv1GAM | GATATCCATCGGACG CATCATNCCNYYRTCYTCRTG |
BacBOXdgRv2GAM | GACCTCATGCTCCAG ADRTGRCARTGRTACATRWA |
BacBOXInfFw | CGTCCGATGGATATCACCG |
BacBOXInfRv | ACGGGTCGGCGGC |
MicMCOInfFw | GCTGCGCCATGAGGAT |
MicMCOInfRv | CAGCGTTTTGCGATCGC |
MicMCOFwbam | AGACGGATCCAGCATTCAACCAGTGGCC |
mgBOXInfFw | GATCGCAAAACGCTGACNTGGGGNTAYAAYGGNWS |
mgBOXInfRv | TCCTCATGGCGCAGCADRTGRCARTGRTACATRWA |
MicMCOFw | ATGAGCATTCAACCAGTGGC |
pMALdelMBPRv | GGCGAGAGCCGAGGC |