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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Construction and characterization of a functional chimeric laccase from metagenomes suitable as a biocatalyst

Fig. 2

SDS-PAGE analysis of recombinant MBP-Lcc120-3 (MBP-Mic-mg-MCO) and Lcc120-3 without MBP (Mic-mg-MCO), which were expressed in E. coli BL21 (DE3). Lane M, molecular weight standards (Pre-stained XL-Ladder; APRO Science, Tokushima, Japan); lanes 1, 2, and 3, crude enzyme solutions from recombinant E. coli (pMAL-c5x/pG-KJE8, pMAL-c5x-Mic-mg-MCO120-3/pG-KJE8, and pMAL-c5x-Mic-mg-MCO120-3ΔMBP/pG-KJE8, respectively); lane 4, purified Lcc120-3

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