Fig. 1From: Construction and characterization of a functional chimeric laccase from metagenomes suitable as a biocatalystAlignment analysis of several MCOs to design the primers for isolating target genes from metagenomes. Amino acid sequences were aligned using ClustalW and conserved amino acids were denoted by white letters on black boxes. The conserved amino acid sequences surrounded by a red square were used for design of each primer. B. subtilis (BsuCosA; GenBank accession No.: CAB12449.1), Actinoalloteichus hymeniacidonis (AhyMCO; AOS64008), Burkholderia cepacia (BceMCO; AFQ47357), Brevibacterium linens (BliBOX; KHS53242), Cyanobacterium aponinum (CapBOX; AFZ53266), Frankia sp. (FrkBOX; ABW14182), Lactobacillus brevis (LbrMCO; EEI71081), L. buchneri (LbuBOX; AEB74417), Mycobacterium tuberculosis (MtbMCO; SGC69794), Nocardia seriolae (NseBOX; APA98799), Rhodococcus rhodochrous (RrhBOX; ETT23846), Streptomyces sp. (StrBOX; AEN08529), Xanthobacter autotrophicus (XauBOX; ABS68135)Back to article page