Table 2 The primers were used to screen the presence of the mecA and genes encoding adhesion factors and biofilm
genes | Primer sequence PCR | product size (bp) | References |
|---|---|---|---|
mecA | 5’-ATCGATGGTAAAGGTTGG-3’ 5’-AGTTCTGCAGTACCGGATTTG-3’ | 533 | (Al-Ali et al. 2014) |
icaA | 5’- TGGCTGTATTAAGCGAAGTC − 3’ 5’- CCTCTGTCTGGGCTTGACC − 3’ | 669 | (Martins et al. 2015) |
icaD | 5’-AAACGTAAGAGAGGTGG-3’ 5’-GGCAATATGATCAAGATAC-3’ | 381 | (Vasudevan et al. 2003) |
bap〹 | 5’-CCC TAT ATC GAA GGT GTA GAA TTG-3’ 5’-GCTGTTGAAGTTAATACTGTACCTGC-3’ | 971 | (Cucarella et al. 2004) |
sarA | 5’-TTAGCTTTGAAGAATTCGCTGT-3’ 5’-TTCAATTTCGTTGTTTGCTTC-3’ | 275 | (Padmapriya et al. 2003) |
agr〹 | 5’-GTAGAGCCGTATTGATTCC-3’ 5’-GTATTTCATCTCTTTAAGG-3’ | 463 | (Moore and Lindsay, 2001) |
16 S rRNA〹 | 5’-GTA GGT GGC AAG CGT TAT CC-3’ 5’-CGC ACA TCA GCG TCA G-3’ | 228 | (Moore and Lindsay, 2001) |