From: CRISPR/Cas9-induced β-carotene hydroxylase mutation in Dunaliella salina CCAP19/18
Primer name | Base sequence (5’-3’) | Purpose |
---|---|---|
T7-sgRNA-F1 | TTAATACGACTCACTATAGGCTAACCCACT GACACCCAGTTTTAGAGCTAGAAATAGC | Transcription in vitro |
T7-sgRNA-F2 | TTAATACGACTCACTATAGGATGCGGGTGA GATGCCTTGGTTTTAGAGCTAGAAATAGC | Transcription in vitro |
T7-sgRNA-F3 | TTAATACGACTCACTATAGGTGCGCAACAA CAGCTACAGGGTTTTAGAGCTAGAAATAGC | Transcription in vitro |
T7 sgRNA-R-common | AAAAGCACCGACTCGGTGCC | Transcription in vitro, colony PCR |
CHY-F | AAGGTCAACACAAGGGAACGA | Target gene Dschyb |
CHY-R | GTTTGGTGTCAGGAAGCCGT |