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Table 2 The primers used for sgRNA transcription in vitro and target genes

From: CRISPR/Cas9-induced β-carotene hydroxylase mutation in Dunaliella salina CCAP19/18

Primer name

Base sequence (5’-3’)

Purpose

T7-sgRNA-F1

TTAATACGACTCACTATAGGCTAACCCACT

GACACCCAGTTTTAGAGCTAGAAATAGC

Transcription in vitro

T7-sgRNA-F2

TTAATACGACTCACTATAGGATGCGGGTGA

GATGCCTTGGTTTTAGAGCTAGAAATAGC

Transcription in vitro

T7-sgRNA-F3

TTAATACGACTCACTATAGGTGCGCAACAA

CAGCTACAGGGTTTTAGAGCTAGAAATAGC

Transcription in vitro

T7 sgRNA-R-common

AAAAGCACCGACTCGGTGCC

Transcription in vitro, colony PCR

CHY-F

AAGGTCAACACAAGGGAACGA

Target gene Dschyb

CHY-R

GTTTGGTGTCAGGAAGCCGT