Table 1 The primers used for different vectors construction
From: CRISPR/Cas9-induced β-carotene hydroxylase mutation in Dunaliella salina CCAP19/18
Primer name | Base sequence (5’-3’) | Vector name |
|---|---|---|
sgRNA1-F | ATTGGGCTAACCCACTGACACCCA | pKSE401-sgRNA1 |
sgRNA1-R | AAACTGGGTGTCAGTGGGTTAGCC | |
sgRNA2-F | ATTGGATGCGGGTGAGATGCCTTG | pKSE401-sgRNA2 |
sgRNA2-R | AAACCAAGGCATCTCACCCGCATC | |
sgRNA3-F | ATTGTGCGCAACAACAGCTACAGG | pKSE401-sgRNA3 |
sgRNA3-R | AAACCCTGTAGCTGTTGTTGCGCA |