Fig. 2From: CRISPR/Cas9-induced β-carotene hydroxylase mutation in Dunaliella salina CCAP19/18Analysis of sgRNA cleavage activity for target DNA. a Cleavage results of three sgRNA-Cas9 complexes to target DNA. b Cleavage results of single three sgRNAs to target DNA. TS: PCR products of target DNA; sgRNA: transcribed RNAs in vitro; Cas9: Cas9 protein; + : The plus represents the component in reaction system. −: The minus shows no this component in reaction systemBack to article page