Table 2 The main primers used in this study
Primers | Sequence |
|---|---|
P1 | CATGCGGCCGCTTAGCTTGCAGTGGGCTTACATGG |
P2 | CGGTCCGGAAACCCCAGAGTCCCGCTCAG |
P3 | CGGTCCGGAGCAGTCATGTCGTGCTAATGTGTAAAAC |
P4 | CCGCTCGAGCATTGTCAACAACAAGACCCATCAT |
P5 | CCGCTCGAGGGGATAACGCAGGAAAGAACATG |
P6 | GCGGGCCCCCTTAACGTGAGTTTTCGTTCCACT |
P7 | CATGCGGCCGCCCAGCTCATAGACCGTATCCAAAG |
P8 | TCCCCCGGGTTTCCTGCGTTATCCCCTGATT |
P9 | CTTGAGCTGTTGACAATTAATCATCGTGTGGTACCATACTAGTGGATCCTTCCTACCC |
P10 | GGGTAGGAAGGATCCACTAGTATGGTACCACACGATGATTAATTGTCAACAGCTCAAGGGCC |
P11 | GATCCTTCCTAGCTAGCTTGGTTGGCGCGCCAATACTTAAGCCC |
P12 | GGGCTTAAGTATTGGCGCGCCAACCAAGCTAGCTAGGAAG |
P13 | CATGCGGCCGCCAGTGGAACGAAAACTCACGTTAAG |
P14 | GCTCCTAGGAGCTCATAGACCGTATCCAAAGCAT |
P15 | GCTCTAGATAGCTTGCAGTGGGCTTACATGG |
P16 | ATTCTCGAGTCACGTAGCGATAGCGGAGTGT |
P17 | ATTCTCGAGCTGGCACGCATAGCCAAGCTAG |
P18 | CATGCGGCCGCCATTGTCAACAACAAGACCCATCAT |
P19 | GCTCTAGAGCCTGGGGTGCCTAATGAGT |
P20 | CATGCCATGGTACAACGTCGTGACTGGGAAAAC |
ldhsg-F | GTGGATCCCAGCATGTAGCGGAATCGAGGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
ldh-U-F | CCGCTCGAGTGCTTCCAGACGGTTTCATC |
ldh-U-R | CACCTTGCGATCATCGACATAAGGGCTCCACTTCCTACGG |
ldh-D-F | CCGTAGGAAGTGGAGCCCTTATGTCGATGATCGCAAGGTG |
ldh-D-R | TTCCTAGCTAGCATCGGCCAAGGTCAAAGTG |
eutDsg-F | CGGGATCCGTCGCCGTTGTGGACCATCAGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
eutD-U-F | CCGCTCGAGACAAATTCATGGGAGGTGCC |
eutD-U-R | AAGCAAGGCAAGCACGTTGGCGCAAGAAGATGCCAGACT |
eutD-D-F | AGTCTGGCATCTTCTTGCGCCAACGTGCTTGCCTTGCTT |
eutD-D-R | GCTCTAGAGCGTAGATTCCTGCTGGACC |
gabPsg-F | GTGGATCCGTGGAGGCATTGATCACCACGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
sgRNA-R | CACGACAGGTTTCCCGACTG |
gabP-F | GTGGTACCATACTAGTGGATCCGTGGAGGCATTGATC |
gabP-U-F | CAGTCGGGAAACCTGTCGTGTGCCACTTTCACCGACTTGG |
gabP-U-R | CACAAACGCCAGGGAGTACAGCAGGGATACTTCGGCGATG |
gabP-D-F | CATCGCCGAAGTATCCCTGCTGTACTCCCTGGCGTTTGTG |
gabP-D-R | GCCAACCAAGCTAGCTAGGAACCGTGATGCTGCCTCTTCTAG |
glnA1sg-F | CGTGGATCCCGTGGAGCATGGTGTTGAAGGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
glnA1-U-F | ATTCTCGAGACAATAGCAATAACCCAGGAAACAC |
glnA1-U-R | AGGAGTTCAGGGTTGCGTTGCGAGGTCTGGCAGGAGATTC |
glnA1-D-F | GAATCTCCTGCCAGACCTCGCAACGCAACCCTGAACTCCT |
glnA1-D-R | TGCTCTAGACATCTGAACTGATCGGCATCTAG |
glnA2sg-F | CGTGGATCCGAAATATCCGCCGTTGTCAGGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
glnA2sg-R | GGATTTGTTGTGGGGCTTGTCACGACAGGTTTCCCGACTG |
glnA2-F | CGTGGATCCGAAATATCCGCCGTTGTCAG |
glnA2-U-F | CAGTCGGGAAACCTGTCGTGACAAGCCCCACAACAAATCC |
glnA2-U-R | CCAGATCCATCGACATTCCATTCGTGTTCCTACCTACCGTTTG |
glnA2-D-F | CAAACGGTAGGTAGGAACACGAATGGAATGTCGATGGATCTGG |
glnA2-D-R | TTCCTAGCTAGCCTCATCGGAGCAGGAGTAAGC |
gdhA1sg-F | CGTGGATCCGCAAACGCCTAGGATGTACAGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
gdhA1-F | GTGGTACCATACTAGTGGATCCGCAAACGCCTAGGATG |
gdhA1-U-F | CAGTCGGGAAACCTGTCGTGTGATGCGGTAGCGGTTCCTTTG |
gdhA1-U-R | GTTCACATCAACCGGCTTGTCATAC |
gdhA1-D-F | GTATGACAAGCCGGTTGATGTGAACGGGGAAGAATTAGGCAGGCATC |
gdhA1-D-R | AAGCTAGACCCGGGCTTAAGCTCACGAAGTAAACGCAGCCGTAG |
alaTsg-F | CGTGGATCCGGTTGCCAGGCTGATGTGCTGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
alaTsg-R | ATTCTCGAGCACGACAGGTTTCCCGACTG |
alaT-U-F | ATTCTCGAGCGGGGTAATGCCATAACGAG |
alaT-U-R | GAATAGCGTGCTGAGCTGGGCGGAATAATGCCTTTGGAGGT |
alaT-D-F | ACCTCCAAAGGCATTATTCCGCCCAGCTCAGCACGCTATTC |
alaT-D-R | TTCCTAGCTAGCGACGCAGCAAGACCTGACATAC |
argRsg-F | CGTGGATCCTCGCGGGCGATGCTATCTACGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
argRsg-R | CAAAGCCAATCATGTAGGAGTTGCACGACAGGTTTCCCGACTG |
argR-F | TGGTACCATACTAGTGGATCCTCGCGGGCGATGCTATCTAC |
argR-U-F | CAGTCGGGAAACCTGTCGTGCAACTCCTACATGATTGGCTTTG |
argR-U-R | CGAGAACGAAAACGGTGTCATAGTTGTACCTGGCTGGTGACTT |
argR-D-F | AAGTCACCAGCCAGGTACAACTATGACACCGTTTTCGTTCTCG |
argR-D-R | TGGCGCGCCAACCAAGCTAGCAACCTGGTCGTTGCCCTTAC |
gabsg-F | CGGGATCCCCAGTAATCAACCCCAGCGAGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
gab-U-F | CCGCTCGAGACGAGTTCGCTGATTTGGATG |
gab-U-R | CAAGTCGGTGAAAGTGGCAACATGGTGAGGTTGGTCCGTC |
gab-D-F | GACGGACCAACCTCACCATGTTGCCACTTTCACCGACTTG |
gab-D-R | GCTCTAGAGGTGCCTAAAACAAAGAATCCAAG |
aceABsg-F | CGTGGATCCGGAAATCCTCGTACGCCTCTGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
aceABsg-R | GCCTCATCGGTGTCGTTGTAACACGACAGGTTTCCCGACTG |
aceAB-F | TGGTACCATACTAGTGGATCCGGAAATCCTCGTACG |
aceAB-U-F | CAGTCGGGAAACCTGTCGTGTTACAACGACACCGATGAGGC |
aceAB-U-R | GTATCCGAGGATGGACTGGCAGGAACTCGGCGCAATGGGCT |
aceAB-D-F | GGAACTCGGCGCAATGGGCT |
aceAB-D-R | TGGCGCGCCAACCAAGCTAGCCCAGGGTTCGCTACGGAATC |
poxBsg-F | GTGGATCCGGTCACCGGATACTTCACCGGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
poxB-F | GTGGTACCATACTAGTGGATCCGGTCACCGGATACTTC |
poxB-U-F | CAGTCGGGAAACCTGTCGTGGTTGCACTGCATGATCGGTT |
poxB-U-R | CGCTGAAGGCTGTGGTGTTT |
poxB-D-F | AAACACCACAGCCTTCAGCGTCGCAGTAACCAGAGCATTCC |
poxB-D-R | AAGCTAGACCCGGGCTTAAGGTTTTCGAGGCGACCAGACAG |
speC-F | ATTCTCGAGTCACATGTTCTTTCCTGCGTTATC |
speC-R | AAGCAAGGCAAGCACGTTGTTACTTCAACACATAACCGTACAACCG |
gdhA-F | AGTCTGGCATCTTCTTGCGCGACGTTTGAGCTGTTGACAATTAATC |
gdhA-R | GAGCTCGAATTCTTAAATCACACC |
gadB-F | CATCGCCGAAGTATCCCTGCATTTTGGGGAAGAATTAGGCAG |
gadB-R | CGCCAGGGAGTACATCAGGTATGTTTAAAGCTGTTCTGTT |