Fig. 2
l-Glu and l-Gln biosynthetic pathway and the results of gene editing. a l-Glu and l-Gln biosynthetic pathway. Pyr: pyruvate; Ac-CoA: acetyl-CoA; Cit: Citrate; Aco: cis-aconitate; Icit: isocitate; α-KG: alpha-ketoglutaramate; Suc-CoA: succinyl-CoA; Suc: succinate; Fum: fumarate; Mal: malate; OAA: oxaloacetate. b The first lane is DNA marker and the second lane is the control before editing, the upstream primer of gdhA1 was designed in promoter PtacM, there is no PCR product in negative cells. c The plasmid stability at different temperature. The cells harboring plasmid were cultured at 25 °C, 28 °C, 30 °C and 37 °C respectively, the total number of colonies were counted by cfu per plate