Table 2 Primers used in this study
Primers | Sequence (5’-3’) | Restriction sites |
|---|---|---|
lysG-PlysE-R | TAATGTCGACTTAAGGCCGCAATCCCTCG | SalI |
lysG-PlysE-F | ACCATCCTATAACTCCTTCTCGGTCCGATGGACAGCAAAAG | Â |
egfp-F | AGAAGGAGTTATAGGATGGTTTCCAAGGGCG | Â |
egfp-R | TTACTTGTACAGCTCGTCC | Â |
cdd-F | AGTAAGAGAGGAGGGATTGCATGACTGAAGATGACTTAGATCTG | Â |
cdd-R | AGTACTGCAGCTAAAGAGCCTTATCCGGAG | PstI |
16Â s-RNA-1 | ACCTGGAGAAGAAGCACCG | Â |
16Â s-RNA-2 | TCAAGTTATGCCCGTATCG | Â |
dapC-1 | TCTTATGTGGGGGCTACACC | Â |
dapC-2 | CATCACGATCCCCAATATCC | Â |
CGB98_RS08395-1 | GGTCCTAACGCTTTCGACTG | Â |
CGB98_RS08395-2 | GCTGTTCAGTGCGTGGATAA | Â |
CGB98_RS10415-1 | TGTCAATAACTCGCCTGGTG | Â |
CGB98_RS10415-2 | CAGTGCATCAACGTGGACTT | Â |
actA-1 | TGCAACCTCCTTCTCCTTGT | Â |
actA-2 | TGCCATAGATGTCAGCTTCG | Â |
CGB98_RS10920-1 | TGCACGTAAAGACGGAGTTG | Â |
CGB98_RS10920-2 | GTTGCCGTAGATGTCGTTGA | Â |