Programming adaptive laboratory evolution of 4-hydroxyisoleucine production driven by a lysine biosensor in Corynebacterium glutamicum

AMB Express

Table 1 Bacterial strains and plasmids used in this study

Strains or plasmids	Description	Source
Strains
JM109	Plasmid propagating strain of E. coli	Novagen
SZ06	Ile-producing strain of C. glutamicum ssp. lactofermentum	Shi et al. 2019
SZ06/pMK	SZ06 harboring pMK	This work
MKn	Positively evolved mutant strains of SZ06/pMK	This work
Kn	Final evolved strains of MKn that lose pMK	This work
Plasmids
pJYW-4	Constitutive expression vector of C. glutamicum, Km^R	Hu et al. 2014
pDTW109	cre-expressing vector of C. glutamicum, temperature-sensitive, Cm^R	Hu et al. 2013
pMK	pDTW109 harboring lysG and P_lysE controlled cdd-egfp genes, Cm^R	This work