Fig. 1

Iturin A inhibiting growth of HepG2 cell. a Effect of iturin A treatment on HepG2 cell viability. The inhibitory effect of iturin A on HepG2 cells was stronger than control. The higher the concentration of iturin A, the weaker the cell viability. Data are represented as the mean of each group + SD. * indicates that the iturin A effect on HepG2 was significantly different compared with the control (n = 3, p < 0.05). (b, c) Chart of cell cycle detection by flow cytometry. Iturin A treatment caused a significant increase in the portion of cells in G2/M phase and decreased the portion of cells in the S phase. The peak of apoptotic cells is indicated by the black arrow in (c). d, e Analysis of apoptotic and necrotic cell populations using flow cytometry. Iturin A treatment significantly promoted the occurrence of apoptosis, especially at the early stage in HepG2 cells