AMB Express

Table 2 Comparison of calculated overall and ∆t_max yields and specific growth rates µ of shake flask cultivations with different relative filling volumes employing strains B. subtilis JABs24 (reference), MG12 (P_srfA::P_narG amyE::[P_narG-lacZ, spcR]) and MG14 (P_srfA::P_nasD amyE::[P_nasD-lacZ, spcR])

From: Evaluation of an oxygen‐dependent self‐inducible surfactin synthesis in B. subtilis by substitution of native promoter P_srfA by anaerobically active P_narG and P_nasD

Relative filling volume	B. subtilis strain	Overall yield to CDW_max
Relative filling volume	B. subtilis strain	Y_P/X [g/g]	Y_X/S [g/g]	Y_P/S [g/g]	q [g/(g h)]	µ [1/h]
10%	JABs24	0.021 ± 0.001	0.216 ± 0.026	0.005 ± 0.001	0.001 ± 0.000	0.144 ± 0.013
	MG12	0.000 ± 0.000	0.175 ± 0.030	0.000 ± 0.000	0.000 ± 0.000	0.223 ± 0.001
	MG14	0.000 ± 0.000	0.239 ± 0.055	0.000 ± 0.000	0.000 ± 0.000	0.185 ± 0.002
50%	JABs24	0.346 ± 0.242	0.129 ± 0.000	0.044 ± 0.031	0.007 ± 0.005	0.116 ± 0.002
	MG12	0.026 ± 0.004	0.183 ± 0.012	0.005 ± 0.001	0.001 ± 0.000	0.130 ± 0.000
	MG14	0.191 ± 0.009	0.181 ± 0.019	0.035 ± 0.002	0.004 ± 0.000	0.122 ± 0.005
100%	JABs24	0.413 ± 0.002	0.090 ± 0.006	0.036 ± 0.002	0.011 ± 0.000	0.111 ± 0.001
	MG12	0.173 ± 0.015	0.120 ± 0.064	0.020 ± 0.010	0.005 ± 0.000	0.126 ± 0.003
	MG14	1.007 ± 0.028	0.051 ± 0.006	0.053 ± 0.005	0.023 ± 0.001	0.079 ± 0.000

Relative filling volume	B. subtilis strain	∆t_max
Relative filling volume	B. subtilis strain	Y_P/X [g/g]	Y_X/S [g/g]	Y_P/S [g/g]	q [g/(g h)]	µ [1/h]
10%	JABs24	1.081 ± 0.039	0.535 ± 0.181	0.194 ± 0.001	0.141 ± 0.008	0.369 ± 0.022
	MG12	0.000 ± 0.000	0.289 ± 0.032	0.000 ± 0.000	0.000 ± 0.000	0.775 ± 0.006
	MG14	0.000 ± 0.000	0.339 ± 0.093	0.000 ± 0.000	0.000 ± 0.000	0.527 ± 0.005
50%	JABs24	1.273 ± 0.095	0.629 ± 0.156	0.217 ± 0.037	0.169 ± 0.002	0.343 ± 0.001
	MG12	0.080 ± 0.007	0.331 ± 0.059	0.304 ± 0.279	0.020 ± 0.002	0.833 ± 0.078
	MG14	0.476 ± 0.005	0.451 ± 0.130	0.629 ± 0.554	0.119 ± 0.001	0.471 ± 0.003
100%	JABs24	0.642 ± 0.015	0.208 ± 0.015	0.099 ± 0.017	0.090 ± 0.001	0.205 ± 0.007
	MG12	0.303 ± 0.030	0.291 ± 0.036	0.030 ± 0.012	0.076 ± 0.008	0.388 ± 0.015
	MG14	1.061 ± 0.179	0.161 ± 0.089	0.243 ± 0.135	0.209 ± 0.012	0.151 ± 0.002

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