Evaluation of an oxygen‐dependent self‐inducible surfactin synthesis in B. subtilis by substitution of native promoter PsrfA by anaerobically active PnarG and PnasD

AMB Express

Table 1 Overview of strains used in the current study

Strain	Genotype or description	References
B. subtilis
JABs24	trp⁺ sfp⁺ ΔmanPA	Geissler et al. (2019b)
MG1	trp⁺ sfp⁺ ΔmanPA amyE::[P_narG-lacZ, spcR]	Hoffmann et al. (2020)
MG5	trp⁺ sfp⁺ ΔmanPA amyE::[P_nasD-lacZ, spcR]	Hoffmann et al. (2020)
KM1016	trp⁺ sfp⁺ ΔmanPA amyE::[P_srfA-lacZ, spcR]	This study
MG11	trp⁺ sfp⁺ ΔmanPA P_srfA::P_narG	This study
MG12	trp⁺ sfp⁺ ΔmanPA P_srfA::P_narG amyE::[P_narG-lacZ, spcR]	This study
MG13	trp⁺ sfp⁺ ΔmanPA P_srfA::P_nasD	This study
MG14	trp⁺ sfp⁺ ΔmanPA P_srfA::P_nasD amyE::[P_nasD-lacZ, spcR]	This study
E. coli
BL21-Gold (DE3)	F^- ompT hsdS(rB^- mB^-) dcm⁺ Tet^r gal λ(DE3) endA Hte	Agilent, Waldbronn, Germany